Ginsenoside Rh2 Inhibits Migration of Lung Cancer Cells under Hypoxia via mir-491

Author(s):

Yingying Chen, Yuqiang Zhang, Wei Song, Ying Zhang, Xiu Dong and Mingqi Tan*   Pages 1633 - 1641 ( 9 )

Abstract:

<P>Background: Ginsenoside Rh2 (Rh2), which is extracted from ginseng, exerts antitumor activity. Here we would like to study the role of Rh2 on hypoxia-induced migration in lung adenocarcinoma. </P><P> Methods: Lung adenocarcinoma A549 and H1299 cells were cultured in 1% O2 condition to mimic the hypoxic tumor microenvironment. The migrations of cancer cells were measured by transwell assay and scratch assay. </P><P> Results: Rh2 could inhibit hypoxia-induced A549 and H1299 cell migration via increase of mir-491 expression. Further, mir-491 antisense oligonucleotide could repress hypoxia-induced migration and the expression of matrix metalloproteinase (MMP)-9 expression in Rh2-treated A549 cells. </P><P> Conclusion: These findings suggest that Rh2 exerts anti-metastasis activity in the hypoxic tumor microenvironment in lung adenocarcinoma cells via mir-491.</P>

Keywords:

Ginsenoside Rh2, hypoxia, lung adenocarcinoma, migration, mir-491, antitumor activity.

Affiliation:

Respiratory Medicine Department, Shengjing Hospital of China Medical University, Shenyang, 110042, Liaoning Province, Respiratory Medicine Department, Shengjing Hospital of China Medical University, Shenyang, 110042, Liaoning Province, Oncology Medicine Department, Shengjing Hospital of China Medical University, Shenyang, 110042, Liaoning Province, Respiratory Medicine Department, Shengjing Hospital of China Medical University, Shenyang, 110042, Liaoning Province, School of Preclinical Medicine, Liaoning University of Traditional Chinese Medicine, 79 Chong Shan Dong Lu, Huanggu District, Shenyang, Liaoning, 110847, Respiratory Medicine Department, Shengjing Hospital of China Medical University, Shenyang, 110042, Liaoning Province

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