Influence of New Synthetic Xanthones on the Proliferation and Migration Potential of Cancer Cell Lines In Vitro

Author(s):

Natalia Szkaradek*, Daniel Sypniewski, Dorota Żelaszczyk, Sabina Gałka, Paulina Borzdziłowska, Henryk Marona and Ilona Bednarek   Pages 1949 - 1965 ( 17 )

Abstract:

<P>Background: Natural plant metabolites and their semisynthetic derivatives have been used for years in cancer therapy. Xanthones are oxygenated heterocyclic compounds produced as secondary metabolites by higher plants, fungi or lichens. Xanthone core may serve as a template in the synthesis of many derivatives that have broad biological activities. </P><P> Objective: This study synthesized a series of 17 new xanthones, and their anticancer potential was also evaluated. </P><P> Methods: The anticancer potential was evaluated in vitro using a highly invasive T24 cancer cell line. Direct cytotoxic effects of the xanthones were established by IC50 estimation based on XTT assay. </P><P> Results: 5 compounds of the total 17 showed significant cytotoxicity toward the studied cancer cultures and were submitted to further detailed analysis, including studies examining their influence on gelatinase A and B expression, as well as on the cancer cells migration and adhesion to an extracellular matrix. These analyses were carried out on five human tumor cell lines: A2780 (ovarian cancer), A549 (lung cancer), HeLa (cervical cancer), Hep G2 (liver cancer), and T24 (urinary bladder cancer). All the compounds, especially 4, showed promising anticancer activity: they exhibited significant cytotoxicity towards all the evaluated cell lines, including MCF-7 breast cancer, and hindered migration-motility activity of cancer cells demonstrating more potent activity than α-mangostin which served as a reference xanthone. </P><P> Conclusion: These results suggest that our xanthone derivatives may be further analyzed in order to include them in cancer treatment protocols.</P>

Keywords:

Xanthones, cancer, cytotoxicity, apoptosis, proliferation, cell migration.

Affiliation:

Department of Bioorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Str., 30-688 Krakow, Department of Biotechnology and Genetic Engineering, Medical University of Silesia, 8 Jednosci Str., 41-200 Sosnowiec, Department of Bioorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Str., 30-688 Krakow, Department of Biotechnology and Genetic Engineering, Medical University of Silesia, 8 Jednosci Str., 41-200 Sosnowiec, Department of Biotechnology and Genetic Engineering, Medical University of Silesia, 8 Jednosci Str., 41-200 Sosnowiec, Department of Bioorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Str., 30-688 Krakow, Department of Biotechnology and Genetic Engineering, Medical University of Silesia, 8 Jednosci Str., 41-200 Sosnowiec

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