Novel Fluorine Boron Hybrid Complex as Potential Antiproliferative Drugs on Colorectal Cancer Cell Line

Author(s):

Yasin Tülüce*, Hawro D.I. Masseh, İsmail Koyuncu, Ahmet Kiliç, Mustafa Durgun and Halil Özkol   Pages 627 - 637 ( 11 )

Abstract:

<P>Objective: Colorectal Cancer (CRC) is one of the most common types of cancer in both sexes; it is considered to be the third leading death factor among other types of cancer. </P><P> This study aimed to examine the cytotoxicity of a new fluorine boron hybrid complex [L(BF2)2] on human colorectal adenocarcinoma cell line (HT-29), based on the potency of the half-metal based complexes to initiate apoptosis. </P><P> Methods: Based on this data, the impact of it in different concentrations on HT-29 cancerous cells was determined by apoptosis (ELISA, DNA fragmentation laddering, AO/EB staining), cytotoxicity (MTT) and genotoxicity (comet assay). We also calculated the cellular Oxidative Stress Index (OSI) by measuring the Total Antioxidant Status (TAS) and Total Oxidant Status (TOS). </P><P> Results: Firstly, [L(BF2)2] was examined in view of cytotoxic effect in seven various cell lines (HELA, DU-145, PC3, DLD-1, ECC, PNT1-A and HT-29), and then it was found that the applied complex had a mighty antiproliferative action on HT-29 cells. Thus, the most effective IC50 value turned out to be 26.49 &#181;M in HT-29 cell line. The present study found a tremendous efficacy of [L(BF2)2] on HT-29 cells, especially in terms of damage to cancer cells&#039; DNA, and consequently caused a series of reactions leading to programmed cell death. </P><P> Conclusion: The results suggest that the [L(BF2)2] as a novel fluorine boron hybrid complex can induce the apoptosis of HT-29 colorectal cancerous cell line and is a possible candidate for future cancer studies.</P>

Keywords:

Antiproliferative, apoptosis, colorectal cancer cytotoxicity, DNA injury, chemotherapy, ELISA.

Affiliation:

Department of Medical Biology, Van Yuzuncu Yil University, Faculty of Medicine, Van, Department of Medical Biology, Van Yuzuncu Yil University, Faculty of Medicine, Van, Department of Biochemistry, Harran University, Faculty of Medicine, S.Urfa, Department of Chemistry, Harran University, Faculty of Science and Art, S.Urfa, Department of Chemistry, Harran University, Faculty of Science and Art, S.Urfa, Department of Medical Biology, Van Yuzuncu Yil University, Faculty of Medicine, Van

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