Turmeric Extract-loaded Selenium Nanoparticles Counter Doxorubicin-induced Hepatotoxicity in Mice <i>via</i> Repressing Oxidative Stress, Inflammatory Cytokines, and Cell Apoptosis

Author(s):

Barakat M. ALRashdi*, Mohamed M. Hussein, Rawan M. Mohammed, Nada W. Abdelhamed, Maran E. Asaad, Mubarak Alruwaili, Saad M. Alrashidi, Ola A. Habotta, Ahmed E. Abdel Moneim* and Shimaa S. Ramadan   Pages 443 - 453 ( 11 )

Abstract:

<p>Background: Doxorubicin (DOX) is an antitumor anthracycline used to treat a variety of malignancies; however, its clinical use is associated with noticeable hepatotoxicity. Therefore, the current study was designed to delineate if biosynthesized SeNPs with turmeric extract (Tur-SeNPs) could alleviate DOX-induced hepatic adverse effects. <p> Methods: Mice were orally post-treated with Tur extract, Tur-SeNPs, or N-acetyl cysteine after the intraperitoneal injection of DOX. <p> Results: Our findings have unveiled a remarkable liver attenuating effect in DOX-injected mice post-treated with Tur-SeNPs. High serum levels of ALT, AST, ALP, and total bilirubin induced by DOX were significantly decreased by Tur-SeNPs therapy. Furthermore, Tur-SeNPs counteracted DOX-caused hepatic oxidative stress, indicated by decreased MDA and NO levels along with elevated levels of SOD, CAT, GPx, GR, GSH, and mRNA expression levels of Nrf-2. Noteworthily, decreased hepatic IL-1&#946;, TNF-&#945;, and NF-&#954;B p65 levels in addition to downregulated iNOS gene expression in Tur-SeNPs-treated mice have indicated their potent antiinflammatory impact. Post-treatment with Tur-SeNPs also mitigated the hepatic apoptosis evoked by DOX injection. A liver histological examination confirmed the biochemical and molecular findings. <p> Conclusions: In brief, the outcomes have demonstrated Tur loaded with nanoselenium to successfully mitigate the liver damage induced by DOX via blocking oxidative stress, and inflammatory and apoptotic signaling.</p>

Keywords:

Nanoselenium, turmeric extract, doxorubicin, hepatotoxicity, apoptosis, oxidative stress.

Affiliation:

Graphical Abstract:

Read Full-Text article