Inactivation of PDH can Reduce Anaplastic Thyroid Cancer Cells’ Sensitivity to Artemisinin

Author(s):

Yitian Li*   Pages 1753 - 1760 ( 8 )

Abstract:

<P>Background: Anaplastic Thyroid Cancer (ATC) is a rare subtype of thyroid tumors with a high mortality rate. Targeted therapies against ATC are ineffective and mostly transient. Artemisinin has shown excellent anti-tumor activity in several cancers, but its effects on ATC are still unknown. <P> Objective: To evaluate the effects of artemisinin on ATC cells and assess the mechanism underlying drug resistance. <P> Methods: The viability and proliferation rates of the artemisinin-treated CAL-62 and BHT-101 cells were analyzed by MTT and EdU incorporation assays. The protein expression levels were determined by Tandem Mass Tag (TMT) labeling quantitative proteomics and western blotting. <P> Results: Artemisinin treatment significantly decreased the expression levels of COX2 and COX7A2 and increased that of COX14, YEM1l1, ALAS1, and OAT after 48h. In addition, FTL was upregulated in the CAL-62 cells and downregulated in BHT-101 cells. The CAL-62 cells showed transient and reversible resistance to artemisinin, which was correlated to time-dependent changes in HIF1&#945;, PDK1, and PDHA levels. <P> Conclusion: Artemisinin targets the mitochondrial respiratory chain proteins in ATC cells. CAL-62 cells show transient resistance to artemisinin via PDH downregulation, indicating that PDH activation may enhance the cytotoxic effects of artemisinin on ATC cells.</P>

Keywords:

Anaplastic thyroid cancer, artemisinin, tolerance, oxidative phosphorylation, HIF1&#945;, PDK1.

Affiliation:

Research Department of Jining Medical University Post Address: He Hua Road 133, Jining Medical University, Jining, Shandong

Graphical Abstract:

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