Nuciferine Inhibits Skin Cutaneous Melanoma Cell Growth by Suppressing TLR4/NF-κB Signaling

Author(s):

Jingxing Xu, Anxin Ying and Tongxin Shi*   Pages 2099 - 2105 ( 7 )

Abstract:

<P>Background: Melanoma causes more than 80% of deaths from all dermatologic cancers. Hence, screening and identifying effective compounds to inhibit the growth of melanoma have crucial importance in basic and clinical treatment. </P><P> Methods: High throughput screening was performed to screen and identify compounds that have anti-melanoma ability. Melanoma cell and mouse allograft models were used to examine the anti-tumor effects of Nuciferine (NCFR). Western blot, qPCR, and lentivirus overexpression were applied to detect the activation of the TLR4/NF-&#954;B signaling pathway. </P><P> Results: NCFR administration significantly suppressed melanoma cell growth and tumor size by inhibiting the phosphorylation of p65. NCFR treatment also could suppress TNF-&#945;-induced activation of NF-&#954;B signaling. The anti-tumor effect of NCFR might be mediated by targeting Toll-like receptors 4. </P><P> Conclusion: NCFR inhibits melanoma cell growth and suppresses tumor size, which provides potential therapeutic strategies for melanoma treatment.</P>

Keywords:

Nuciferine, NF-&#954;B, TLR4, p65, melanoma, qPCR.

Affiliation:

Department of Dermatology, Qingdao Municipal Hospital, No.1. Jiaozhou Road, Qingdao 266011, Shandong, Department of Dermatology, Qingdao Municipal Hospital, No.1. Jiaozhou Road, Qingdao 266011, Shandong, Department of Dermatology, Qingdao Municipal Hospital, No.1. Jiaozhou Road, Qingdao 266011, Shandong

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