Erinacerins, Novel Glioma Inhibitors from <i>Hericium erinaceus</i>, Induce Apoptosis of U87 Cells through Bax/Capase-2 Pathway

Author(s):

Feng Zhang, Hui Lv* and Xuhua Zhang*   Pages 2082 - 2088 ( 7 )

Abstract:

<P>Background: Glioma is the most common tumor of the central nervous system. Hericium erinaceus, which has been reported to have a variety of pharmacological activities, is a widely used Traditional Chinese Medicine (TCM), and also a kind of delicious food accepted by the public. </P><P> Methods and Results: In this study, two new natural products, compounds 1 and 2, were isolated and identified from Hericium erinaceus. They were named erinacerin O and erinacerin P, respectively, after the structural identification, and their effects on human glioma cell line U87 were evaluated. Erinacerin P (2) exhibited obvious cytotoxicity on human glioma cell line U87. The IC50 value of 2 was 19.32μg/mL. The results showed that the apoptosis of U87 cells treated with 2 increased and the morphology of U87 cells altered significantly. Flow cytometry experiment showed that 2 could significantly increase the apoptosis rate of U87 cells and reduce DNA replication. Western blot results suggested the Bax/capase-3 pathway was involved in the U87 cell apoptosis induced by 2. </P><P> Conclusion: Erinacerin O and Erinacerin P are novel compounds obtained from Hericium erinaceus and Erinacerin P could be a potential novel glioma inhibitor.</P>

Keywords:

Hericium erinaceus, erinacerin, U87, cell viability, apoptosis, nuclear magnetic resonance.

Affiliation:

Department of Neurosurgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, Department of Neurology, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032, Department of Critical Care Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110032

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