Radioiodinated Ginger Compounds (6-gingerol and 6-shogaol) and Incorporation Assays on Breast Cancer Cells

Author(s):

Kadriye B. Karatay , Ayfer Yurt Kılçar*, Emine Derviş and Fazilet Z. Biber Müftüler   Pages 1129 - 1139 ( 11 )

Abstract:

<P>Background: 6-Gingerol (6G) and 6-Shogaol (6S) are the main active components of ginger. 6-Gingerol is known for its anti-metastatic and anti-invasive pharmacological activities on cancer cells, besides, 6-Shogaol also inhibits breast cancer cell invasion. </P><P> Objective: In this study, radioiodination (¹³¹I) of 6G and 6S was aimed. Additionally, it is aimed to monitor their incorporation behavior on breast cancer cell lines. </P><P> Methods: 6-Gingerol was isolated from the fresh ginger-roots extract, additionally, dehydrated to obtain 6-Shogaol. 6G and 6S were radioiodinated using iodogen method. Quality control studies of radioiodinated ginger compounds (6G and 6S) were performed by thin layer radio-chromatography. In vitro studies of radioiodinated ginger compounds on MCF-7 and MDA-MB-231 cells were performed with incorporation assays. </P><P> Results: 6-Gingerol and 6-Shogaol were radioiodinated (¹³¹I-6G and ¹³¹I-6S) in high yields over 95%. ¹³¹I-6S demonstrated higher incorporation values than ¹³¹I-6G on MDA-MB-231 cells. Incorporation behavior of ¹³¹I-6G and ¹³¹I-6S was similar to MCF-7 cells. </P><P> Conclusion: It has been observed that ginger compounds were radioiodinated successfully and ¹³¹I-6S have a noteworthy incorporation on MDA-MB-231 cells which is a known breast carcinoma cell line with highly invasive characteristics.</P>

Keywords:

6-gingerol, 6-shogaol, radioidination, iodine-131, breast cancer cell lines, radio-chromatography.

Affiliation:

Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir, Department of Nuclear Applications, Institute of Nuclear Sciences, Ege University, 35100 Bornova, Izmir

Graphical Abstract:

Read Full-Text article