Effects of Somatostatin and Vitamin C on the Fatty Acid Profile of Breast Cancer Cell Membranes

Author(s):

Aysegul Hanikoglu, Ertan Kucuksayan, Ferhat Hanikoglu, Tomris Ozben*, Georgia Menounou, Anna Sansone, Chrys Chatgilialoglu, Giuseppe Di Bella and Carla Ferreri   Pages 1899 - 1909 ( 11 )

Abstract:

<p> Background: Vitamin C (Vit C) is an important physiological antioxidant with growing applications in cancer. Somatostatin (SST) is a natural peptide with growth inhibitory effect in several mammary cancer models. </p><p> Objective: The combined effects of SST and Vit C supplementation have never been studied in breast cancer cells so far. </p><p> Methods: We used MCF-7 and MDA-MB231 breast cancer cells incubated with SST for 24h, in the absence and presence of Vit C, at their EC50 concentrations, to evaluate membrane fatty acid-profiles together with the follow-up of EGFR and MAPK signaling pathways. </p><p> Results: The two cell lines gave different membrane reorganization: in MCF-7 cells, decrease of omega-6 linoleic acid and increase of omega-3 fatty acids (Fas) occurred after SST and SST+Vit C incubations, the latter also showing significant increases in MUFA, docosapentaenoic acid and mono-trans arachidonic acid levels. In MDA-MB231 cells, SST+Vit C incubation induced significant membrane remodeling with an increase of stearic acid and mono-trans-linoleic acid isomer, diminution of omega-6 linoleic, arachidonic acid and omega-3 (docosapentaenoic and docosadienoic acids). Distinct signaling pathways in these cell lines were studied: in MCF-7 cells, incubations with SST and Vit C, alone or in combination significantly decreased EGFR and MAPK signaling, whereas in MDA-MB231 cells, SST and Vit C incubations, alone or combined, decreased p- P44/42 MAPK levels, and increased EGFR levels. </p><p> Conclusion: Our results showed that SST and Vit C can be combined to induce membrane fatty acid changes, including lipid isomerization through a specific free radical-driven process, influencing signaling pathways.

Keywords:

Somatostatin, Vitamin C, membrane fatty acid profile, breast cancer, cell signaling, gas chromatography.

Affiliation:

Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Department of Biochemistry, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, Department of Biochemistry, Faculty of Medicine, Biruni University, Istanbul, Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Consiglio Nazionale delle Ricerche, ISOF, Via Piero Gobetti 101, 40129 Bologna, Consiglio Nazionale delle Ricerche, ISOF, Via Piero Gobetti 101, 40129 Bologna, Consiglio Nazionale delle Ricerche, ISOF, Via Piero Gobetti 101, 40129 Bologna, Di Bella Foundation, Via G. Marconi 51, 40122 Bologna, Consiglio Nazionale delle Ricerche, ISOF, Via Piero Gobetti 101, 40129 Bologna

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