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Anticancer Potential of Doxorubicin in Combination with Green-Synthesized Silver Nanoparticle and its Cytotoxicity Effects on Cardio-Myoblast Normal Cells

[ Vol. 21 , Issue. 14 ]

Author(s):

Jafar Saeidi, Samaneh Dolatabadi, Mahbubeh B. Esfahani, Masood Saeidi, Mahnaz Mohtashami, Kiana Mokhtari and Ahmad Ghasemi*   Pages 1842 - 1849 ( 8 )

Abstract:


Background: The use of DOX as an anticancer agent is associated with serious side effects on normal cells especially in cardiovascular tissue.

Objective: Here, it was proposed suggested the combination of low dose of DOX with AgNPs provide ideal cytotoxicity against cancer cells and decrease side effects against normal human cells. This study evaluated evaluates the cytotoxic effects of green-synthetized AgNPs (GS-AgNPs) in combination with DOX in cancerous cells (MCF7) and investigated investigates its influences on cell growth and apoptosis in a normal cell line of heart (H9c2).

Methods: We used of coffee extracts, as a reducing and stabilizing agent, for green-synthesis of AgNPs. GS-AgNPs were characterized by using various analytical methods. MTT assay was used for cell toxicity analysis in cancerous and normal cells. Moreover, Annexin-V /PI staining and mRNA expression of Bax, Bcl2 and p53 were performed for apoptosis measurement in heart normal cell line.

Results: GS-AgNPs showed more biocompatibility for normal cells and higher cytotoxicity for cancerous cells compared to that reported for chemical synthesized nanoparticles. Our results also demonstrated that a selected combination of DOX and AgNPs, 20 µM AgNPs / 0.3 µM DOX, has a suitable cytotoxic effect against cancerous cells with a minimum toxic effect on normal cells. So, no significant alteration was observed in cell migration capacity, apoptosis and gene expression of BAX, Bcl-2 and P53 when H9c2 cells were treated with 20 µM AgNPs / 0.3 µM DOX relative to the non-treated control.

Conclusion: Finally, it seems that the combination of GS-AgNPs and DOX could be a potent strategy to combat cancer.

Keywords:

Green-synthesized silver nanoparticle, cytotoxicity, combination therapy, cancer, doxorubicin, gene expression.

Affiliation:

Department of Physiology, School of Basic Science, Neyshabur Branch, Islamic Azad University, Neyshabur, Department of Biology, School of Basic Science, Neyshabur Branch, Islamic Azad University, Neyshabur, Department of Biology, School of Basic Science, Shiraz Branch, Islamic Azad University, Shiraz, Faculty of Pharmacy, Tabriz University of Medical Science, Tabriz, Department of Biology, School of Basic Science, Neyshabur Branch, Islamic Azad University, Neyshabur, Department of Biology, School of Basic Science, Neyshabur Branch, Islamic Azad University, Neyshabur, Department of Basic Sciences, Neyshabur University of Medical Sciences, Neyshabur



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