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Derivatives of Deoxypodophyllotoxin Induce Apoptosis through Bcl-2/Bax Proteins Expression

[ Vol. 21 , Issue. 5 ]

Author(s):

Ya-Nan Li, Ni Ning, Lei Song, Yun Geng, Jun-Ting Fan, Chao-Ying Ma* and He-Zhong Jiang*   Pages 611 - 620 ( 10 )

Abstract:


Background: Deoxypodophyllotoxin, isolated from the Traditional Chinese Medicine Anthriscus sylvestris, is well-known because of its significant anti-tumor activity with strong toxicity in vitro and in vivo.

Objective: In this article, a series of deoxypodophyllotoxin derivatives were synthesized and their anti-tumor effectiveness was evaluated.

Methods: The anti-tumor activity of deoxypodophyllotoxin derivatives was investigated by the MTT assay method. Apoptosis percentage was measured by flow cytometer analysis using Annexin-V-FITC.

Results: The derivatives revealed obvious cytotoxicity in the MTT assay by decreasing the number of late cancer cells. The decrease of Bcl-2/Bax could be observed in MCF-7, HepG2, HT-29, and MG-63 using Annexin V-FITC. The ratio of Bcl-2/Bax in the administration group was decreased, which was determined by the ELISA kit.

Conclusion: The derivatives of deoxypodophyllotoxin could induce apoptosis in tumor cell lines by influencing Bcl-2/Bax.

Keywords:

Deoxypodophyllotoxin derivatives, tumor cell, flow cytometer, Bcl-2/Bax, Anthriscus sylvestris, anti-tumor activity.

Affiliation:

School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, Department of Pharmaceutical Analysis, School of Pharmacy, Nanjing Medical University, Nanjing, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031



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