Fahimeh H. Beigi, Soheil Fatahian, Sogand Shahbazi-Gahrouei, Daryoush Shahbazi-Gahrouei* and Amin Farzadniya Pages 1918 - 1926 ( 9 )
Objective: Polydopamine coated iron oxide nanoparticles (Fe3O4@PDA NPs) were synthesized, characterized, and their MR imaging contrast agents and photothermal potency were evaluated on melanoma (B16-F10 and A-375) cells and normal skin cells. To this end, MTT assay, Fe concentration, and MR imaging of both coated and uncoated NPs were assessed in C57BL/6 mice.Methods: Fe3O4 nanoparticles were synthesized using co-precipitation, and coated with polydopamine. The cytotoxicity of Fe3O4 and Fe3O4@PDA NPs on melanoma cells, with different concentrations, were obtained using MTT assay. MR images and Fe concentrations of nanoprobe and nanoparticles were evaluated under in vivo conditions. Results: Findings indicated that uncoated Fe3O4 showed the highest toxicity in animal (B16-F10) cells at 450μg/ml after 72h, while the highest toxicity in human (A-375) cells were observed at 350μg/ml. These nanoparticles did not reveal any cytotoxicity to normal skin cells, despite having some toxicity features in A-375 cells. MR image signals in the tumor were low compared with other tissues. The iron concentration in the tumor was higher than that of other organs. Conclusion: It is concluded that the cytotoxicity of Fe3O4@PDA was found to be significantly lower than uncoated nanoparticles (p <0.001), which allows some positive effects on reducing toxicity. The prepared nanoprobe may be used as a contrast agent in MR imaging.
Fe3O4, Fe3O4@PDA NPs, B16-F10, A-375, cytotoxicity, MR imaging.
Falavarjan Branch, Islamic Azad University, Isfahan, Department of Biophysics, Falavarjan Branch, Islamic Azad University, Isfahan, Faculty of Humanities Najafabad Branch, Islamic Azad University, Isfahan, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Department of Radiology, Askarieh Hospital, Isfahan