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Chlorambucil Cytotoxicity Reduction in Rats Through Bone Marrow, An In vivo Study

[ Vol. 18 , Issue. 15 ]

Author(s):

Ebtesam H.O. Nafie*, Eman Khater, Mohamed Awwad, Mohamed Zowail and Kholud Hegazy   Pages 2187 - 2192 ( 6 )

Abstract:


Background: Bone Marrow (BM) has the self-renovation capacity and has been used recently in tumor medicine. Chlorambucil [CHB] is ordinarily utilized chemotherapy to treat varieties of malignancy patients. This investigation intended to gauge the effectiveness of BM as an in-vivo antimutagenic against CHB.

Methods: The experimental design relies upon four classes; each class contains ten adult male albino rats as follows: control, rats infused orally with CHB for fourteen days, rats intravenously infused with BM through a tail vein one time, rats infused the mix of CHB and BM.The Anticancer capability of BM was assessed by cytogenetic assay and mitotic index. The declarations of the apoptosis- related genes were examined by RT-qPCR examination.

Results: The present experiment demonstrated a curative effect of BM against the cytotoxic impact of CHB. Infusion of BM after chemotherapy helps to diminish the chromosomal aberration; increment mitotic index and decline the Bax/Bcl2 proportion compared with [CHB] class gather that prompts expanding the survival rate of influenced cells with chemotherapy cytotoxicity.

Conclusion: The present study shows that bone marrow transplantation together after CHB infusion helps to increase genomic stability by diminishing structural chromosome abnormalities, diminishing the Bax/Bcl2 proportion and increasing the mitotic index.

Keywords:

Chlorambucil, bone marrow, chromosomal aberration, BAX, BCL-2, RT-qPCR.

Affiliation:

Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Department of Zoology, Faculty of Science, Benha University, Benha, 13518, Department of Zoology, Faculty of Science, Benha University, Benha, 13518

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