Apoptotic Effect of Gemini Curcumin on MDA-MB-468 Breast Cancer Cell Line

Author(s):

Masoumeh Hajizadeh, Salar Hafez Ghoran, Hewa Jalal Azeez, Mohammad Ali Hosseinpour Feizi and Esmaeil Babaei*   Pages 2181 - 2188 ( 8 )

Abstract:

<p>Background: Gemini Curcumin (Gemini-Cur) is the latest nanoformulation of curcumin with a significant apoptotic effect on cancer cell lines. <p> Objective: This in vitro study aims to evaluate the apoptotic effects of Gemini-Cur toward MDA-MB-468 breast cancer cell lines and further the related mechanism of apoptosis. <p> Methods: The cytotoxicity of Gemini-Cur toward MDA-MB-468 cell lines was tested using MTT assay. Furthermore, the expression ratio of Bax/Bcl-2 was evaluated by qRT-PCR. Consequently, the protein expression of Bax/Bcl-2, survivin, and caspase-3 was measured using western blotting. <p> Results: Having treated MDA-MB-468 cell lines with Gemini-Cur, the IC₅₀ values were found to be 44.44 and 31.63 μM at 24 and 48 h, respectively. Our findings showed that Gemini-Cur significantly suppresses cancer cell proliferation in a time- and dose-dependent manner. Furthermore, the data revealed that curcumin nanoformulation induces apoptosis in MDA-MB-468 cells through modulation of the expression of Bax, Bcl-2, Survivin, and Caspase-3. <p> Conclusion: The data of the current study propose that Gemini-Cur can be considered a promising candidate against triple-negative breast cancer.</p>

Keywords:

Apoptosis, breast cancer, Bax/Bcl-2 ratio, gemini curcumin, MDA-MB-468, survivin, caspase-3.

Affiliation:

Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Department of Chemistry, Faculty of Science, Golestan University, Gorgan, Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz

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