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Is Quercetin Beneficial for Colon Cancer? A Cell Culture Study, Using the Apoptosis Pathways

[ Vol. 22 , Issue. 1 ]

Author(s):

Meltem Özgöçmen*, Dilek Bayram, İlkay Armağan, Gülçin Yavuz Türel, Murat Sevimli and Nurgül Şenol   Pages 193 - 200 ( 8 )

Abstract:


Background: Quercetin (QCT) is a dietary flavonoid with many beneficial effects (<i>e.g</i>., antioxidant, antiaging, antidiabetic, antifungal effects, and regulation of gastrointestinal motor activity in human); furthermore, it induces apoptosis, cell cycle arrest, and differentiation. <p> Objective: The apoptotic effects of OCT were investigated on SW480 human colon cancer cell lines in monolayer and spheroid cultures. <p> Methods: Quercetin (40–200 μM) was applied, and Inhibitory Concentration (IC50) doses were determined for three time intervals (24, 48, and 72 h). The effective dose was determined and applied for analyses, including staining with BrdU to investigate cell proliferation, terminal deoxynucleotidyl transferase dUTP nick and labeling (TUNEL) to investigate apoptosis, and caspase-3 and Apoptosis Inducing Factor (AIF) to investigate caspase-dependent or independent apoptotic pathways. <p> Results: The effective dose of QCT was determined to be 200 μM and was found to induce apoptosis and inhibit cell proliferation at 24, 48, and 72 h,both in 2D and 3D cultures. Significant increases were observed in both caspase-3 and AIF staining, but cells showed greater caspase-3 staining compared with AIF staining at all time intervals (p<0.05). <p> Conclusion: The QCT treatment groups showed more cell death and less cell growth compared with the untreated control groups in both 2D and 3D cultures of SW480 cell lines. The results suggest that quercetin induces apoptosis, inhibits cell proliferation, and has a protective role against colon cancer. However, further studies are needed to clarify its mechanism of action.

Keywords:

AIF, caspase 3, monolayer culture, quercetin, spheroid culture, SW480.

Affiliation:

Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Department of Medical Biology, Faculty of Medicine, Süleyman Demirel Universtiy, Isparta, Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Department of Nutrition Sciences, Faculty of Healty Sciences, Süleyman Demirel University, Isparta

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