Anti-oxidant and Anticancerous Effect of <i>Fomitopsis officinalis</i> (Vill. ex Fr. Bond. et Sing) Mushroom on Hepatocellular Carcinoma Cells <i>In Vitro</i> through NF-kB Pathway

Author(s):

Nyamsambuu Altannavch, Xi Zhou, Md. Asaduzzaman Khan, Ashfaque Ahmed, Shinen Naranmandakh, Jun-Jiang Fu and Han-Chun Chen*   Pages 1561 - 1570 ( 10 )

Abstract:

<P>Background: Fomitopsis officinalis (Vill. ex Fr. Bond. et Sing) is a medicinal mushroom, commonly called ‘Agarikon’; it has traditionally been used to treat cough and asthma in the Mongolian population. <P> Objective: The objective of the study was to examine the significance of biological activity of F. officinalis and evaluation of the antioxidant activity and anticancer activity of six fractions of F. officinalis residues (Fo1-powder form dissolved in ethanol, Fo2-petroleum ether residue, Fo3-chloroformic, Fo4-ethylacetate, Fo5-buthanolic, and Fo6-waterethanolic) against hepatocellular carcinoma cells. <P> Methods: We performed in vitro studies of cell proliferation and viability assay, annexin V-FITC/Propidium Iodide assay, and NF-kB signaling pathway by immunoblot analysis. <P> Results: Our findings revealed that all six fractions/extracts have antioxidant activity, and somehow, they exert anticancerous effects against cancer cells. In cancerous cell lines (HepG2 and LO2), Fo3 chloroformic extract promoted the cancer cell apoptosis and cell viability, activated G2/M-phase cell cycle, and selectively induced NF-kB proteins, revealing as a novel antitumor extract. <P> Conclusion: This study reports that Fo3-chloroformic extract is rich in antitumor activity, which was previously not investigated in cancer. To develop the impact of F. officinalis among natural products to treat/prevent oxidative stress disorders or cancers, further examinations of F. officinalis are needed to develop new natural drugs to treat cancer. However, this study assessed only one extract, Fo3-chloroformic, which has a significant impact against cancer cell lines.</P>

Keywords:

Fomitopsis officinalis, antioxidant activity, apoptosis, anticancer, HepG2, NF-kB signaling pathway.

Affiliation:

Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, 172 Tongzipo Road, Changsha, Hunan 410013, Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, 172 Tongzipo Road, Changsha, Hunan 410013, Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, 172 Tongzipo Road, Changsha, Hunan 410013, Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, 172 Tongzipo Road, Changsha, Hunan 410013, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar 14201, Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan 646000, Department of Biochemistry and Molecular Biology, School of Life Sciences, Central South University, School of Life Sciences, Central South University, 172 Tongzipo Road

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