A Novel 4H-Chromen-4-One Derivative from Marine <i>Streptomyces ovatisporus</i> S4702^T as Potential Antibacterial and Anti-Cancer Agent

Author(s):

Aslıhan Kurt-Kızıldoğan*, Neslihan Akarsu, Çiğdem Otur, Arif Kivrak, Nevroz Aslan-Ertas, Sevki Arslan, Dogukan Mutlu, Metin Konus, Can Yılmaz, Dogan Cetin, Tufan Topal and Nevzat Şahin   Pages 362 - 370 ( 9 )

Abstract:

Background: Marine actinomycetes are among indispensable sources of natural bioactive compounds with unique antimicrobial and anti-cancer activities. <p> Objective: Herein, it was aimed to elucidate the bioactive potential of a marine-derived <i>Streptomyces ovatisporus</i> S4702T, isolated previously. <p> Methods: <i>Streptomyces ovatisporus</i> S4702^T was cultured in N-Z Amine broth, and extraction was carried out using different organic solvents. Bioassay-guided purification was followed by chemical characterization using NMR and LC-MS/MS. The compound was then evaluated for its antibacterial, antioxidant and cytotoxic activities. <p> Results: Etyl acetate extracts gave the highest antibacterial activity, and chemical characterization of this extract indicated the formula as C15H29O5N3 and the corresponding possible molecular structure as 4H-chromen-4-one derivative. It was found highly potent against <i>Bacillus subtilis</i> ATCC 6633 (MIC: 0.25 μg ml^-1) and <i>Micrococcus luteus</i> ATCC 9341 (MBC: 0.5 μg ml^-1). It has no remarkable antioxidant activity, but a higher EC50 value and less cytotoxicity against normal cells. The EC50 values of this chromen derivative were found as 9.68 μg ml-1 for human colon carcinoma, 9.93 μg ml^-1 for human prostate adenocarcinoma and 25.5 μg ml^-1 for human embryonic kidney cells. <p> Conclusion: Overall, the presented 4H-chromen-4-one derivative is a remarkable bioactive compound with potent antibacterial and cytotoxic activity. With its high bioactive potential, it is proposed as a good candidate in medicine.

Keywords:

<i>Streptomyces ovatisporus</i> S4702^T, 4H-chromen-4-one, antibacterial activity, NMR, LC-MS/MS, cytotoxicity.

Affiliation:

Department of Agricultural Biotechnology, Ondokuz Mayıs University, 55139, Samsun, Department of Agricultural Biotechnology, Ondokuz Mayıs University, 55139, Samsun, Department of Agricultural Biotechnology, Ondokuz Mayıs University, 55139, Samsun, Department of Chemistry, Van Yuzuncu Yil University, 65080, Van, Department of Chemistry, Van Yuzuncu Yil University, 65080, Van, Department of Biology, Pamukkale University, 20100, Denizli, Department of Biology, Pamukkale University, 20100, Denizli, Department of Molecular Biology and Genetics, Van Yuzuncu Yil University, 65080, Van, Department of Molecular Biology and Genetics, Van Yuzuncu Yil University, 65080, Van, Department of Molecular Biology and Genetics, Van Yuzuncu Yil University, 65080, Van, High Technology Application and Research Center, Pamukkale University, 20020, Denizli, Department of Molecular Biology and Genetics, Ondokuz Mayıs University, 55139, Samsun

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