A Comparison of the Anti-Cancer Effects of Free and PLGA-PAA Encapsulated Hydroxytyrosol on the HT-29 Colorectal Cancer Cell Line

Author(s):

Nasrin S. Sani, Habib Onsori*, Somayeh Akrami and Mohammad Rahmati   Pages 390 - 394 ( 5 )

Abstract:

Background: Hydroxytyrosol is one of the phenolic compounds of olive oil and can induce anticancer effects on colorectal cancer cells. <p> Objective: The aim of the present study was to evaluate the free hydroxytyrosol and nano-capsulated hydroxytyrosol effects on the cell cycle arrest in HT-29 colorectal cancer cell line. <p> Methods: The nano-capsulated hydroxytyrosol was synthesized in poly lactide-co-glycolide-co-polyacrylic acid (PLGA-PAA) copolymer. MTT assay was performed to evaluate the anti-proliferative and anti-tumor effects of the free hydroxytyrosol and nano-capsulated hydroxytyrosol. Finally, the relative expression of <i>CDKN1A</i>, <i>CDKN1B</i>, and <i>CCND1</i> genes was evaluated in control and treated colorectal cancer cells by using Real-Time PCR. <p> Results: The obtained results from the MTT assay showed that the cytotoxic effects of the nano-capsulated hydroxytyrosol on the colorectal cancer cell line (IC50= 6PPM) were significantly more than free hydroxytyrosol (IC50= 12PPM) after 72h. Also, nano-capsulated hydroxytyrosol showed more significant effects on the upregulation of <i>CDKN1A</i> and <i>CDKN1B</i> genes and down-regulation of the <i>CCND1</i> gene in colorectal cancer cells. <p> Conclusion: In conclusion, the present study showed that hydroxytyrosol led to the death of colorectal cancer cells through cell cycle arrest. Also, the PLGA-PAA copolymer dramatically caused to increase the cytotoxic effects of the hydroxytyrosol on the colorectal cancer cells.

Keywords:

Colorectal cancer, PLGA-PAA copolymer, hydroxytyrosol, HT-29, CCND1, CDKN1A, CDKN1B.

Affiliation:

Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Department of Cell and Molecular Biology, Marand Branch, Islamic Azad University, Marand, Department of Biology, Marand Branch, Islamic Azad University, Marand, Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz

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