Antimutagenic, Antiproliferative and Antioxidant Properties of Sea Grape Leaf Extract Fractions (<i>Coccoloba uvifera</i> L.)

Author(s):

Jorge A. Ramos-Hernández, Montserrat Calderón-Santoyo, Armando Burgos-Hernández, Joel S. García-Romo, Arturo Navarro-Ocaña, María G. Burboa-Zazueta, Edgar Sandoval-Petris and Juan A. Ragazzo-Sánchez*   Pages 2250 - 2257 ( 8 )

Abstract:

<P>Background: Cancer is a disease characterized by the invasion and uncontrolled growth of cells. One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field in this area. </P><P> Objective: In this study biological potential of 11 fractions from Coccoloba uvifera L. leaf hexane extract was evaluated by several in vitro tests. </P><P> Methods: Leaves were lyophilized and hexane extraction was performed. The extract was fractionated by column chromatography with hexane, ethyl acetate, and methanol. The antimutagenic (Ames test), antiproliferative (MTT test), and antioxidant capacity (DPPH, ABTS, and ferrous ion chelation) of the fractions were evaluated. </P><P> Results: Fractions 4, 6, 8, and 9 have antimutagenic activity (against sodium azide in strain TA100), fraction 11 showed antiproliferative capacity (IC₅₀ of 24 ± 9 μg/mL in cells of HCT 116). The fractions with the highest activity were analyzed by HPLC-MS and lupeol, acacetin, and &#946;-sitosterol were identified. </P><P> Conclusion: This study demonstrates, for the first time, the bioactivity of C. uvifera leaf as a new source of High Biological Value Compounds (HBVC), which can be of interest to the food and pharmaceutical industries.</P>

Keywords:

Coccoloba uvifera leaf, hexane extract, fractions, antimutagenicity, antioxidant, antiproliferation.

Affiliation:

Laboratorio Integral de Investigacion en Alimentos, Tecnologico Nacional de Mexico-Instituto Tecnologico de Tepic, Av. Tecnologico 2595, C.P. 63175 Tepic, Nayarit, Laboratorio Integral de Investigacion en Alimentos, Tecnologico Nacional de Mexico-Instituto Tecnologico de Tepic, Av. Tecnologico 2595, C.P. 63175 Tepic, Nayarit, Departamento de Investigacion y Posgrado en Alimentos, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Departamento de Investigacion y Posgrado en Alimentos, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Universidad Nacional Autonoma de Mexico, Facultad de Quimica, Circuito Interior, Ciudad Universitaria, Col. Copilco, C.P. 04510 Coyoacan, Ciudad de Mexico, Departamento de Investigaciones Científicas y Tecnologicas, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Departamento de Investigaciones Científicas y Tecnologicas, Universidad de Sonora, C.P. 8300 Hermosillo, Sonora, Laboratorio Integral de Investigacion en Alimentos, Tecnologico Nacional de Mexico-Instituto Tecnologico de Tepic, Av. Tecnologico 2595, C.P. 63175 Tepic, Nayarit

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