Alpha-Terpineol as Antitumor Candidate in Pre-Clinical Studies

Author(s):

Helber A. Negreiros, Kariely Gonçalves de Moura, Maria L.L. Barreto do Nascimento, Débora Caroline do Nascimento Rodrigues, Paulo M.P. Ferreir, Débora C. Braz, Marlene Gomes de Farias, Layde de Sousa Corrêia, Ana R.S. Pereira, Lubna K.B. Santos, Juan C.R. Gonçalves, Anderson N. Mendes, Felipe C. Carneiro da Silva, Ana A.C.M. Cavalcant and Joáo Marcelo de Castro e Sousa*   Pages 2023 - 2031 ( 9 )

Abstract:

<p>Background: Alpha-terpineol is monoterpene alcohol with anti-tumor activity against different tumor cell lines (lung, breast, leukemias and colorectal) through blockage of NF-kB expression, which play an important role in tumor cells growth. </P><P> Objective: Evaluate the antitumor activity of alpha-terpineol in murine Sarcoma 180 cell line. </P><P> Methods: For the tests, different cytotoxic and genotoxic assays were used, including Trypan blue, cytokinesis- blocked micronucleus assay, comet assay, agarose gel DNA fragmentation, flow cytometry and cell viability using fluorescence. Ascitic fluid cells from sarcoma 180 were obtained from Mus musculus peritoneal cavity and Alpha-terpineol was tested at 100, 250 and 500 μg/mL. Doxorubicin and Cisplatin were used as positive controls. </P><P> Results: Cytotoxic effects of alpha-terpineol were found in all concentrations tested, reducing cell viability in 50.9; 38.53; 30.82% at 100, 250 and 500 μg/mL, respectively. Alpha-terpineol induced genotoxic effects due to DNA fragmentation (increased frequency and index of damage), and was clastogenic by increased micronuclei formation, nucleoplasmic bridges and nuclear buds. DNA fragmentation and increased cell death indicated that alpha-terpineol can cause early, late, and necrotic apoptosis. </P><P> Conclusion: Our data indicate that alpha-terpineol has antitumor activity revealed by cytogenetic mechanisms and / or loss of cell membrane integrity.</p>

Keywords:

Natural products, essential oils, monoterpene, alpha-terpineol, genotoxicity, sarcoma 180.

Affiliation:

Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics and Antitumor Evaluation Laboratory (TOXGEN), Senator Helvídio Nunes Campus of Barros-Picos-PI, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui, Laboratory of Experimental Cancerology - LabCancer, Federal University of Piaui, Teresina, Piaui, Laboratory of Experimental Cancerology - LabCancer, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics and Antitumor Evaluation Laboratory (TOXGEN), Senator Helvídio Nunes Campus of Barros-Picos-PI, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics and Antitumor Evaluation Laboratory (TOXGEN), Senator Helvídio Nunes Campus of Barros-Picos-PI, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics and Antitumor Evaluation Laboratory (TOXGEN), Senator Helvídio Nunes Campus of Barros-Picos-PI, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui, Department of Biochemistry and Pharmacology, Federal University of Piaui, Teresina-PI, 64049-550, Piaui, Department of Physiology and Biophysics, Graduate Programs in Chemistry Federal University of Piaui, Teresina, Piaui, Toxicological Genetics and Antitumor Evaluation Laboratory (TOXGEN), Senator Helvídio Nunes Campus of Barros-Picos-PI, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui, Toxicological Genetics Research Laboratory (LAPGENIC), Postgraduate Program in Pharmaceutical Sciences, Federal University of Piaui, Teresina, Piaui

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