Antiproliferative and Genotoxic Action of an Underexploited Organoteluran Derivative on Sarcoma 180 Cells

Author(s):

Maria L.L. Barreto do Nascimento, Antonielly Campinho dos Reis, José V.O. Santos, Helber A. Negreiros, Felipe C. Carneiro da Silva, Paulo M.P. Ferreira, Juan C.R. Gonçalves, Dalton Dittz, Débora C. Braz, Adriana M.V. Nunes, Rodrigo L.O.R. Cunha, Ana A.C. Melo-Cavalcante and João Marcelo de Castro e Sousa*   Pages 1019 - 1026 ( 8 )

Abstract:

<p>Background: The search for novel metallic chemical compounds with toxicogenic effects has been of great importance for more efficient cancer treatment. </P><P> Objective: The study evaluated the cytotoxic, genotoxic and mutagenic activity of organoteluran RF07 in the S-180 cell line. </P><P> Methods: The bioassays used were cell viability with 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test, evaluation of apoptosis and necrosis using fluorescence and flow cytometry, cytokinesisblock micronucleus test and comet assay. The compound was tested at 1; 2.5 and 5μM. </P><P> Results: The results showed the cytotoxicity of RF07 at concentrations of 2.5, 5, 10 and 20μM when compared to the negative control. For genotoxicity tests, RF07 showed effects in all concentrations assessed by increased index and frequencies of damage and mutagenic alterations. The compound was also cytotoxic due to the significant decrease in the nuclear division index, with significant values of apoptosis and necrosis. The results of fluorescence and flow cytometry showed apoptosis as the main type of cell death caused by RF07 at 5μM, which is thought to avoid an aggressive immune response of the organism. </P><P> Conclusion: In addition to cytotoxic and genotoxic effects, RF07 creates good perspectives for future antitumor formulations.</p>

Keywords:

Chemotherapy, organometals, antitumor activity, DNA damages, MTT, RF07.

Affiliation:

Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina, Department of Biology, Federal University of Piaui, Picos, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina, Department of Pharmaceutical Sciences, Federal University of Paraiba, Joao Pessoa, Department of Biochemistry and Pharmacology, Federal University of Piaui, Teresina, Department of Pharmacy, University of Piaui, Teresina, Department of Biophysics and Physiology, Laboratory of Experimental Cancerology, Federal University of Piaui, Teresina, Center for Natural and Human Sciences, Laboratory of Chemical Biology, Federal University of ABC, Santo Andre, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Toxicological Genetics, Federal University of Piaui, Teresina

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